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Mycoplasma are one of the most common, yet elusive, contaminants of mammalian cell cultures. As the smallest known free-living organism, mycoplasma are a pervasive, parasitic species of highly-infectious bacteria that are estimated to contaminate between 15-35% of all continuous cell cultures worldwide.
Captivate Bio offers an easy-to-use EZ-PCR™ Mycoplasma Detection Kit which is designed for highly-sensitive routine screening and detection of mycoplasma contamination as well as other closely related species. This EZ-PCR™ Mycoplasma Detection Kit is used to detect over 90 different species, to monitor cell line health, to monitor contamination in shared lab spaces, and to rule out that mycoplasma is not interfering with your experiments.
Eight total reactions, including six samples, one negative control, and one positive control were tested, each of which contain the internal control to rule out PCR inhibition (i.e. false negatives). As shown, Sample 4 produced a mycoplasma-positive band at 270bp in addition to the internal control band at 357bp, while Samples 1, 2, 3, 5, and 6 produced only internal control bands and are thus negative for mycoplasma. Image courtesy of WiCell Research Institute.
1 Item
Highly-sensitive PCR assay designed for the simple and fast detection of mycoplasma contamination in cell cultures. Includes ready-to-use reagents to perform 20 tests, each with internal control.
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